TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2016.05.007

Abstract

Abstract:

The aim of this study was to investigate the association between the TXNRD1 gene polymorphisms and the sheep growth traits，and to find the molecular markers related to the growth traits of Ujumqin sheep.The variations in the exons of TXNRD1 gene were detected using DNA pool technology.Then Sequenom Mass-array technology was used to detect the genotypes at the mutation sites of the TXNRD1 gene in 343 Ujumqin sheep.The polymorphisms of TXNRD1 gene and its association with growth traits of Ujumqin sheep were analyzed by SPSS 22.0 software.The results showed that the C→T missense mutation (RS10) in exon 1，the A→C synonymous mutation（RS17） in exon 10 and the T→C synonymous mutation（RS18） in exon 12 were found，respectively.The x² test showed that all the 3 sites were in Hardy-Weinberg equilibrium(P>0.05).Then linkage disequilibrium and haplotypes were analyzed，the results showed that RS10-RS17，RS10-RS18 were strongly linked，while RS17-RS18 were weakly linked.The dominant haplotype was CAT with a frequency of 0.356.Assocition analysis suggested that 3 genotypes at each mutation site had significant difference in 4-month-age weight and chest girth (P<0.05).The 3 genotypes of RS10 showed significant difference in 6-month-age weight，body height，chest girth and chest width(P<0.05).At RS17 site，the 3 genotypes also had significant difference in 6-month-age body height (P<0.05).At RS18 site，there were significant difference among the 3 genotypes in 6-month-age weight，body height，body length and chest girth(P<0.05).Meanwhile，the different combined genotypes showed significant difference in various Ujumqin growth traits(P<0.05).Therefore，the results suggest that TXNRD1 mutation sites maybe markers related to the Ujumqin sheep growth traits and can be used as candidate markers in Ujumqin sheep molecular breeding.

CLC Number:

S826
S813.3

MA Xiao-meng，XUAN Jun-li，WANG Hui-hua，YUAN Ze-hu，WU Ming-ming，ZHU Cai-ye，LIU Rui-zao，WEI Cai-hong，ZHAO Fu-ping，ZHANG Li，DU Li-xin. TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 909-921.

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TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep

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